Genome-Wide Profiling of Histone Modification with Chip-Seq (CD Genomics)

Genome-Wide Profiling of Histone Modification with Chip-Seq (CD Genomics)

reference: CD genimics, Genom-Wide Profiling of Histone Modifications with ChIP-Seq
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Introduction to Histone Modifications

There are at least 9 different types of post-translational modifications (PTM) have been found.

Examples of Modeification	Functions of PTM
methylation acetylation phosphorylation ubiquitylation sumoylation	Change chromatin structures Recruit histone modifiers change in gene expression Transcriptional regulation chromosome packaging ** DNA damage/repair**

Major Types of Modification

1. Methylation

2. Acetylation

3. Phosphorylation

4. Ubiquitylation

ubiquitin moiety (76-amino-acid peptide.)

5. Sumoylation

CHIP-SEQ DATA ANALYSIS