Understanding PacBio Sequencing: A Deep Dive for RNA-Seq Enthusiasts

The blog post delves into the realm of PacBio sequencing, elucidating its significance in the world of next-generation sequencing. Contrasting PacBio with other sequencing technologies, such as Illumina's short-read and Oxford Nanopore's long-read sequencing, the article highlights the unique advantages and challenges posed by each. The comprehensive PacBio data analysis pipeline is elucidated step by step, from raw data collection to final report generation. A special section is dedicated to comparing tools used in the PacBio pipeline, offering insights into their strengths and limitations.