Fly crossover

Fly Crossover

Met_ {RNAi} （III）
Gre_ {RNAi} （III）
Hnf4_ {RNAi} （III）
UAS-GFP （II）

Target:

• $;\frac{UAS_ {GFP}}{CyO}；\frac{Hnf4_ {RNAi}}{TM_ 6b}$

• $Sp/CyO；Met_ {RNAi} /TM₆b$

• $Sp/CyO；Gre_ {RNAi} /TM₆b$

Line 1

$$
;\frac{+}{+};\ Hnf4_ {RNAi}\ \ \ \times\ \ \ ;\frac{Sp}{CyO};\ \frac{Dr}{TM_ 6b}
$$

F₁:

• $;\frac{Sp}{+};\ \frac{Dr}{Hnf4_ {RNAi}}$
• $;\frac{CyO}{+};\ \frac{Dr}{Hnf4_ {RNAi}}$
• $;\frac{Sp}{+};\ \frac{Hnf4_ {RNAi}}{TM_ 6b}$
• $;\frac{CyO}{+};\ \frac{Hnf4_ {RNAi}}{TM_ 6b}$

Line 2

$$
;UAS_ {GFP}; \ \ \times \ \ ;\frac{Sp}{CyO};\frac{Dr}{TM_ 6b}
$$

F₁:

• $;\frac{UAS_ {GFP}}{Sp};\frac{Dr}{+}$
• $;\frac{UAS_ {GFP}}{Sp};\frac{TM_ 6b}{+}$
• $;\frac{UAS_ {GFP}}{CyO};\frac{Dr}{+}$
• $;\frac{UAS_ {GFP}}{CyO};\frac{TM_ 6b}{+}$

L₂F₂:

$$ ;\frac{UAS_ {GFP}}{CyO};\frac{Dr}{+} \ \ \times
\ \ ;\frac{UAS_ {GFP}}{CyO};\frac{TM_ 6b}{+}$$

• Ch2:
  • $\frac{UAS_ {GFP}}{CyO}$; $UAS_ {GFP}$
  • $CyO$ Dead
• Ch3:
  • $\frac{Dr}{+}$; $\frac{CyO}{+}$; $\frac{Dr}{CyO}$

---

• $;UAS_ {GFP};\frac{Dr}{+}$
• $;\frac{UAS_ {GFP}}{CyO};\frac{Dr}{TM_ 6b}$
• $;\frac{UAS_ {GFP}}{CyO};\frac{+}{+}$
• $;\frac{CyO}{CyO};\frac{TM_ 6b}{+}$

L1F₁; L2F₂ crosses

$$
;\frac{UAS_ {GFP}}{CyO};\frac{Dr}{CyO}
\ \ \times\ \ s
$$

$$
R^2 = 0.9889
$$

$$
y = -28.1452x + 135.5281
$$

x = (y - 135.5281) / (-28.1452)