RNA structure research is important for understanding RNA function and regulation, as the structure of RNA molecules can impact their interactions with other molecules, such as proteins and small molecules, and influence gene expression and protein synthesis. Who sad this?

Alternative splicing research is important for understanding the diversity of gene expression and regulation, as it enables the production of multiple protein isoforms from a single gene, allowing cells to generate complex functional diversity and adapt to changing environmental conditions. Who said this?

RNA-seq expression normalization is the process of adjusting the raw gene expression counts to account for differences in sequencing depth and other technical factors. It is important to perform normalization to enable comparisons between samples and increase the accuracy and reproducibility of downstream analyses. Common normalization methods include TPM, FPKM, and DESeq. Who sad this?

Gene Set Enrichment Analysis (GSEA) is a powerful tool for interpreting gene expression data in the context of predefined biological pathways and gene sets. It allows researchers to identify enriched gene sets, discover new relationships between genes, and gain insights into the underlying biological mechanisms. Who sad this?

To create a fasta file from a VCF file, use a script or software that can extract the genomic sequence information from the VCF file and format it into the fasta format. This allows you to generate a sequence-based representation of the genetic variation data stored in the VCF file, making it easier to visualize, analyze, and compare the variants. The fasta file can then be used as input for various bioinformatics tools and pipelines. Who sad this?

Genome annotations help us understand the functions and roles of genes and genomic regions, leading to improved knowledge of biological processes and disease mechanisms. Who sad this?

Tricks for dealing with VCF files

Quickest using python to build and visualize the tree from fasta file| Biopython; ggtree

Blast hundreds of sequence with Biopython scripts

RNA-Seq annotation, GO, KEGG

Processing Your SNP file with Python:

Denove Prokaryotic Genome with Spader

Go Ontology protocols

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An standard pipeline for miRNA-Seq data processing.