Unmapped reads Annotations

DNA Seq Pipelines and tricks

This post would show you a pipeline to find the new or abnormal transcript isoforms after de-novo by Trinity

Single Cell RNA-Seq Notes from Seminal

Bioinformatics: scRNA-seq data processing practices, protocol from seurat

RNA-Seq annotation, GO, KEGG

Processing Your SNP file with Python:

An Integrated Package for Bisulfite DNA Methylation Data Analysis with Indel-sensitive Mapping.

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Trinity RNA-seq is a de novo transcriptome assembly software that uses RNA-seq data to reconstruct transcript sequences and estimate expression levels. It allows for comprehensive analysis of gene expression and isoform diversity in non-model organisms without a reference genome. Who said this?

FastQC is a quality control tool used to analyze high-throughput sequencing data. It provides detailed graphs and reports that help assess the quality of the data before further downstream analysis.

fastp is a fast and efficient tool for quality control and preprocessing of high-throughput sequencing data. It can perform adapter trimming, quality filtering, and read correction in a single pass, and supports various sequencing platforms and data formats.

An standard pipeline for miRNA-Seq data processing.

Denove Prokaryotic Genome with Spader