It would be easy to count the result when we have only a few cells in an image. But once you got thousands of cells in an image and/or you got hundreds of repeats, the work would be tedious and laboring. But with the help of python, we can do more than sample counts and gray intensity calculation. We can apply more complicated techniques like Vironoi spacial calculation and Delaunay triangulation. I'll show how can we apply these two algorithms to finally determine whether cells may share boundaries or be physically contacted.
When we compare our control with another group, the t-test could fit our goal very well. But when we need to compare it into more than 1 group, 3 groups for example, the t-test could only give the random false positive independently. That means three false positives in three comparisons. This would cause a problem. Because the false positive we need to consider now is at least one false positive in three comparisons. It means we need to adjust the p-value and apply a rigorous method to achieve a more reliable result. For doing that, ANOVA was introduced and applied.